User-friendly Software with Ample Functions and Capabilities

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Optimal parameters for flow rate, run time, fraction volume, etc. will be calculated and set automatically upon selecting a column on Yamazen’s “Green Flash” software. The default parameters will be shown in System Setting window.

Yamazen’s Software provides the maximum sample load information for the selected column

Choose a column, click the [Sample load] button, input TLC Rf values of the target compound and the nearest impurity, and the maximum sample load will be calculated and given automatically. If a different column is selected, the maximum sample load will change accordingly. It will be a great guide for you to decide the proper column for running your sample.

State-Of-The-Art Software Based On True Theory of Chromatography

Yamazen is the one and only company that have successfully transferred the method from TLC to column chromatography, based on the true theory of chromatography.

Green Flash Chromatography

     Linear gradient is set with Rf value on TLC. (Rf gradient)

     The optimum method can be automatically developed to elute the target compound at around 4-Column Volume.

     The eluting position of the target compound(s) is shown by yellow arrow(s).

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Sample Eluting Position and Resolution Can Be Fully Controlled for Yamazen Flash Chromatography Systems.

(Patent No.4087395)

2-Step Gradient separates multiple compounds efficiently.

Compared with the conventional ISOCRATIC method, 2-Step Gradient method shortens run time considerably, saves solvents and energy, and boosts productivity, therefore, It is eco-friendly.

Automatic Method Setup for Reverse Phase Chromatography

A small amount of sample is run first for a preliminary run, since there is no correlation between TLC and column chromatography. When the preliminary run ends, click the peak of the target compound. The optimum Gradient method for a real run will be automatically developed, like the one for the normal phase chromatography.

Automatic method setting is possible with various solvents, for example, hexane/toluene.

(For more information, see Yamazen technical bulletin no.61.)

With the use of an Inject (Loading) Column, sample loading can be easily done without any sample loss. No cross contamination.

Benefit of Using an Inject Column for Sample Loading (1)

Inject Column will make sample bands narrower to achieve a higher resolution. Moreover, a larger size sample can be separated by small size column with use of the Inject Column.

Benefit of Using an Inject Column for Sample Loading (2)

Method to Achieve Good Separation of Sample Dissolved in Highly Polar Solvent

No “Coelute”!

Highly polar solvent used to dissolve the sample often causes poor sample separation in flash chromatography. It is, therefore, advisable to use low polar solvent to dissolve the sample. Many times, however, it is necessary to use highly polar solvent when dissolving the tough-to-dissolve sample. Yamazen’s Inject Column eliminates the harmful effect caused by highly polar solvent used to dissolve the sample, by trapping the highly polar solvent. By using Inject column together with main separation column such as Universal column, Hi-Flash column, etc. available from Yamazen, good sample separation can be achieved.

A New Innovative Software to Decide Recommended Size of Inject Column that Eliminates Harmful Effect on Chromatography Caused by Highly Polar Solvent Used to Dissolve Sample (…. will be available soon.)

Input the solvent name and the amount of the solvent used to dissolve a sample, and the recommended size of Inject column that eliminates the harmful effect caused by highly polar solvent used to dissolve the sample, will be decided automatically and pop up in the window.

Broad Detecting Range of Yamazen UV Detector
No Sample Loss! No Peak Saturation!

Detecting capability over a broad range is critically important in flash chromatography!

Yamazen UV detector is capable of detecting a sample with very high sensitivity. As the chromatograms below shows, these samples are accurately detected and beautifully separated at 0.08 AUFS and 0.04 AUFS on a Yamazen UV detector. Ordinary UV detectors of other makes, however, lack the sensitivity, thus, end up losing precious samples.

Sample: Butyl p-hydroxybenzoate, 0.5mg Methyl p-hydroxybenzoate, 0.5mg Toluene, 9mg
Column used: Yamazen’s Hi-Flash, L (30 gram) Fractionation mode: Peak mode separation

Sample: Butyl p-hydroxybenzoate, 500mg Methyl p-hydroxybenzoate, 500mg Toluene, 900mg
Column used: Yamazen’s Hi-Flash, 2L (45 gram)　　　Fractionation mode: Peak mode separation

It’s possible to get a slow gradient from 1% to 7% of polar solvent.

A solvent delivery pump is of high precision medium pressure valveless ceramic piston pump, featuring infinite fine flow adjustments. The gradient at low concentration of solvent as low as 1 to 7% is possible, which is required when using a high polar solvent, like methanol, without pre-mixing.

Effective ceramic piston movement for maximum solvent and bubble clearing, assuring accurate solvent delivery. Bubbles produced by solvents that have low boiling points like dichloromethane will not affect pump.

Real Time Pressure Monitor

The precision pressure mater makes it possible to do real time monitoring of the pressure building up inside the column. When pressure hits the preset limit, the pump will stop as quickly as a flash, which will protect the system from being damaged. Highly responding pressure moderation system (PMS) prevents disposable plastic column and glass column from bursting, and allows the sample run to continue.

Yamazen’s motto is “Safety First!”

Parallel Detection of UV Detector and RI Detector or ELSD

Yamazen RI-31 RI detector and ELSD-100X evaporative light-scattering detector are designed for use with” Smart Flash” purification systems to purify organic compounds with little or no chromophores such as Carbohydrates, Steroids, Lipids, Terpenes and other compounds that have no UV absorbance. Parallel detection between UV detector and RI detector or ELSD is possible with Yamazen flash chromatography system.

System Specifications

* For more specifications of the PC, please inquire.

Standard Accessories

A pair of the same size rack

FR-360 Rack
118Wx335Lx159mmH

Note: FR-360 fraction collector holds a pair of the same size racks.
When ordering the rack, please specify it with Cat. Number.

Rack Specifications, Collection Tube (O.D. x Length x Quantity)

Note: Test tube with * is of Japanese standard.